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Human Metastasis-suppressor KiSS-1 (KISS1) ELISA Kit

Human Metastasis-suppressor KiSS-1 (KISS1) ELISA Kit

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Specification

Product name Human Metastasis-suppressor KiSS-1 (KISS1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Metastasis-suppressor KiSS-1 (KISS1) ELISA Kit employs a two-site sandwich ELISA to quantitate KISS1 in samples. An antibody specific for KISS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKISS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KISS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KISS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative KISS1; KiSS-1; METASTIN; MGC39258; kisspeptin; malignant melanoma metastasis-suppressor

Product Properties

Kit components • Human Metastasis-suppressor KiSS-1 microplate
• Human Metastasis-suppressor KiSS-1 standard
• Human Metastasis-suppressor KiSS-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Metastasis-suppressor KiSS-1 (KISS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KISS1. No significant cross-reactivity or interference between Human KISS1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Kisspeptin is a G-protein coupled receptor ligand for GPR54. Kiss1 was originally identified as a human metastasis suppressor gene that has the ability to suppress melanoma and breast cancer metastasis. It is recently become clear that kisspeptin-GPR54 signaling has an important role in initiating GnRH secretion at puberty, the extent of which is an area of ongoing research.Kisspeptin is a metastasis suppressor of melanomas and breast cancers without affecting tumorigenicity. The encoded protein may function to inhibit chemotaxis and invasion, attenuating metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A polymorphism in the terminal exon of this mRNA results in two protein isoforms.
Gene ID 3814
Alternative KISS1; KiSS-1; METASTIN; MGC39258; kisspeptin; malignant melanoma metastasis-suppressor
Accession Q15726

Image & description

Fig.1. Human Metastasis-suppressor KiSS-1 (KISS1) Standard Curve.

Fig.1. Human Metastasis-suppressor KiSS-1 (KISS1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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