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Human Mannma binding lectin receptor (MBLR) ELISA Kit

Human Mannma binding lectin receptor (MBLR) ELISA Kit

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Specification

Product name Human Mannma binding lectin receptor (MBLR) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Mannma binding lectin receptor (MBLR) ELISA Kit employs a two-site sandwich ELISA to quantitate MBLR in samples. An antibody specific for MBLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMBLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBLR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBLR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MBLR

Product Properties

Kit components • Human Mannma binding lectin receptor microplate
• Human Mannma binding lectin receptor standard
• Human Mannma binding lectin receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Mannma binding lectin receptor (MBLR) ELISA Kit has high sensitivity and excellent specificity for detection of Human MBLR. No significant cross-reactivity or interference between Human MBLR and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PCGF6 contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified.Mouse and human MBLR share 87% sequence identity, and the central domain of MBLR shares 38% identity with the corresponding regions of MEL18 and BMI1. A minor transcript of 4.5 kb was also expressed in some tissues. RT-PCR confirmed ubiquitous expression.
Alternative MBLR

Image & description

Fig.1. Human Mannma binding lectin receptor (MBLR) Standard Curve.

Fig.1. Human Mannma binding lectin receptor (MBLR) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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