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Human M-CSF protein

Human M-CSF protein

Views(134) Publications(1) Catalog no(PRP100268)
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Specification

Product name Human M-CSF protein
Sequence Amino acid sequence derived from human CSF1 (NP_757349.1) (Met1-Asn190) was expressed with an initial Met.
Activity Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1.6-6.3 ng/mL.
Protein length The recombinant human CSF1 consists 158 amino acids and predicts a molecular mass of 18.4 kDa.
Preparation method HEK293 Cells
Purity > 85 % as determined by SDS-PAGE.
Alternative CSF-1; M-CSF; MCSF; Macrophage colony-stimulating factor 1; CSF1

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Molecular weight 18.4 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human M-CSF protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human M-CSF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.
Gene ID 1435
Alternative CSF-1; M-CSF; MCSF; Macrophage colony-stimulating factor 1; CSF1
Others The Endotoxin level is less than 1.0 EU per μg protein as determined by the LAL method.
Accession NP_757349.1

Image & description

Fig.SDS-PAGE analysis of Human M-CSF protein.

Fig.SDS-PAGE analysis of Human M-CSF protein.

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