- Product name
Human Leptin protein
Amino acid sequence derived from mature form of human Leptin (NP_000221.1) (Val 22-Cys 167) was expressed, with an additional Met.
Measured by its binding ability in a functional ELISA . 1. Immobilized human Leptin at 1.25 μg/ml (100 μl/well) can bind human Leptin receptor Fc chimera with a linear range of 0.032-4.0 μg/ml . 2. Immobilized human Leptin at 5 μg/ml (100 μl/well) can bind human Leptin receptor his with a linear range of 0.032-4.0 μg/ml.
- Protein length
The recombinant human leptin consists of 147 amino acids and predicts a molecular mass of 16 kDa. The apparent molecular mass of rhLEP is approximately 13 kDa in SDS-PAGE under reducing conditions.
- Preparation method
> 98 % as determined by SDS-PAGE
Lyophilized from sterile PBS, pH 7.4
- Molecular weight
- Usage notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human Leptin protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
- Storage instructions
Lyophilized Human Leptin protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
The product is shipped at ambient temperature.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Leptin is one of the most important hormones secreted by adipocytes, as an adipokine that modulates multiple functions including energy homeostasis, thermoregulation, bone metabolism, endocrine and pro-inflammatory immune responses. The circulating leptin levels serve as a gauge of energy stores, thereby directing the regulation of energy homeostasis, neuroendocrine function, and metabolism. Recent studies suggest that leptin is physiologically more important as an indicator of energy deficiency, rather than energy excess, and may mediate adaptation by driving increased food intake and directing neuroendocrine function to converse energy, such as inducing hypothalamic hypogonadism to prevent fertilization. One of these functions is the connection between nutritional status and immune competence. The adipocyte-derived hormone Leptin has been shown to regulate the immune response, innate and adaptive response, both in normal and pathological conditions. Thus, Leptin is a mediator of the inflammatory response. Leptin has a dual effect on bone, acting by two independent mechanisms. As a signal molecule with growth factor characteristics, leptin is able to stimulate osteoblastic cells and to inhibit osteoclast formation and activity, thus promoting osteogenesis. However, as a molecule which stimulates sympathetic neurons in the hypothalamus, leptin indirectly inhibits bone formation. This inhibitory effect of leptin mediated by activation of sympathetic nervous system can be abrogated by application of blood pressure-reducing beta-blockers, which also inhibit receptors of hypothalamic adrenergic neurons. Leptin appears to regulate a number of features defining Alzheimer's disease (AD) at the molecular and physiological level. Leptin can stimulate mitogenic and angiogenic processes in peripheral organs. Because leptin levels are elevated in obese individuals and excess body weight has been shown to increase breast cancer risk in postmenopausal women. Furthermore, a recent report clearly shows that targeting leptin signaling may reduce mammary carcinogenesis.
- Gene ID
- Alternative names
LEPD; OB; OBS, LPT
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Preparation methods for Biochemical
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Antibody application protocols
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- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
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- ♦ Handling and storage suggestion for peptides and protein
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Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
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