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Human Killer cell immunoglobulin-like receptor 2DS4 (KIR2DS4) ELISA Kit

Human Killer cell immunoglobulin-like receptor 2DS4 (KIR2DS4) ELISA Kit

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Specification

Product name Human Killer cell immunoglobulin-like receptor 2DS4 (KIR2DS4) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Killer cell immunoglobulin-like receptor 2DS4 (KIR2DS4) ELISA Kit employs a two-site sandwich ELISA to quantitate KIR2DS4 in samples. An antibody specific for KIR2DS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIR2DS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIR2DS4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIR2DS4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative KIR2DS4; XXbac-BPG230H20.3; CD158I; KIR1D; KIR412; KKA3; MGC120019; MGC125315; MGC125317; NKAT8; KIR antigen 2DS4; killer inhibitory receptor 4-1-2; natural killer cell inhibitory receptor

Product Properties

Kit components • Human Killer cell immunoglobulin-like receptor 2DS4 microplate
• Human Killer cell immunoglobulin-like receptor 2DS4 standard
• Human Killer cell immunoglobulin-like receptor 2DS4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Killer cell immunoglobulin-like receptor 2DS4 (KIR2DS4) ELISA Kit has high sensitivity and excellent specificity for detection of Human KIR2DS4. No significant cross-reactivity or interference between Human KIR2DS4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Using primers known to amplify HLA-C-specific NK receptors to screen clones with NK triggering activity, Bottino et al. (1996) also isolated a KIR2DS4 cDNA, which they termed KKA3. Sequence analysis revealed that KIR2DS4 encodes a deduced 304-amino acid protein with an extracellular domain similar to that of KIR2D p58 receptors; however, in the transmembrane region, there is a charged lysine residue, and the cytoplasmic tail has only 39 amino acids with no ITIM (immunoreceptor tyrosine-based inhibitory motif). KIRs with short cytoplasmic tails are associated with NK cell triggering rather than inhibition. Immunoprecipitation analysis showed that KIR2DS4 is expressed in 2 forms, one of 35 to 45 kD and the other of 55 to 58 kD. Flow cytometry analysis showed that KIR2DS4, unlike KIR2DS1 and KIR2DS2, was expressed in only a minority of individuals tested.
Gene ID 3809
Alternative KIR2DS4; XXbac-BPG230H20.3; CD158I; KIR1D; KIR412; KKA3; MGC120019; MGC125315; MGC125317; NKAT8; KIR antigen 2DS4; killer inhibitory receptor 4-1-2; natural killer cell inhibitory receptor
Accession P43632

Image & description

Fig.1. Human Killer cell immunoglobulin-like receptor 2DS4 (KIR2DS4) Standard Curve.

Fig.1. Human Killer cell immunoglobulin-like receptor 2DS4 (KIR2DS4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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