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Human Kallikrein-12 (KLK12) ELISA Kit

Human Kallikrein-12 (KLK12) ELISA Kit

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Specification

Product name Human Kallikrein-12 (KLK12) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Kallikrein-12 (KLK12) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK12 in samples. An antibody specific for KLK12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative KLK12; DKFZp686H1078; KLK-L5; KLKL5; MGC42603; kallikrein 12; kallikrein-like protein 5

Product Properties

Kit components • Human Kallikrein-12 microplate
• Human Kallikrein-12 standard
• Human Kallikrein-12 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Kallikrein-12 (KLK12) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLK12. No significant cross-reactivity or interference between Human KLK12 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Kallikrein-12 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in three transcript variants encoding different isoforms.In the second mRNA form, the last exon is split into 2 separate exons with an additional intervening intron, producing a deduced 254-amino acid protein with a predicted molecular mass of 27.1 kD. In the third mRNA form, the fourth exon is missing, producing a deduced 111-amino acid protein with a molecular mass of 12 kD. RT-PCR demonstrated that KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord.
Gene ID 43849
Alternative KLK12; DKFZp686H1078; KLK-L5; KLKL5; MGC42603; kallikrein 12; kallikrein-like protein 5
Accession Q9UKR0

Image & description

Fig.1. Human Kallikrein-12 (KLK12) Standard Curve.

Fig.1. Human Kallikrein-12 (KLK12) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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