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Human IL36 alpha protein

Product Information

  • Product name

    Human IL36 alpha protein

  • Sequence

    Amino acid sequence derived from Human IL36 alpha (Q9UHA7) (Lys6-Phe158) was expressed.

  • Protein length

    The recombinant Human IL36 alpha consists of 153 amino acids and has a predicted molecular mass of 17 KD.

  • Preparation method

    E. coli

  • Purity

    >98% as determined by SDS-PAGE

Fig. SDS-PAGE analysis of Human IL36 alpha protein, N-His tag.

Product Properties

  • Formulation

    Lyophilized from sterile PBS, pH 7.4.

  • Features & Benefits

    Endotoxin: < 0.1 EU per µg of the protein as determined by the LAL method.

  • Molecular weight

    17 KD

  • Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL36 alpha protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

  • Storage instructions

    Lyophilized Human IL36 alpha protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

  • Shipping

    The product is shipped at ambient temperature.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Human IL36 alpha previously called IL-1F6 and FIL1 epsilon (family of IL-1 member epsilon), is a member of the IL-1 family which includes IL-1b, IL-1a, IL-1ra, IL-18, IL-36ra (IL1F5), IL-36b (IL1F8), IL-36g (IL1F9), IL-37 (IL1F7) and IL-38 (IL-1F10). IL36a is a pro-inflammatory cytokine which plays an important role in innate and adaptive immunity. IL36a is expressed in the immune system and the fetal brain, but not in other tissues or multiple hematopoietic cell lines.IL-36 alpha, beta, and gamma all activate NF-kappa B and MAPK pathways in an IL‑1 Rrp2 dependent manner, and induce production of inflammatory cytokines and chemokines such as CXCL8/IL-8. IL-36 alpha and other family members are overexpressed in psoriatic skin lesions, and transgenic overexpression of IL‑36 alpha in skin keratinocytes produces epidermal hyperplasia.

  • Gene ID

    27179

  • Alternative names

    Interleukin-36 Alpha; FIL1 Epsilon; Interleukin-1 Epsilon; IL-1 Epsilon; Interleukin-1 Family Member 6; IL-1F6; IL36A; FIL1E; IL1E; IL1F6

  • Accession

    Q9UHA7

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Human IL36 alpha protein
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: PRP1027

  • Size
  • Price
  • 10µg $48
  • 50µg $120
  • 100µg $170
  • 1mg $800

Contact information

Do not hesitate to contact us if you have any questions.

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