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Human IL-9 protein, His Tag

Human IL-9 protein, His Tag

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Specification

Product name Human IL-9 protein, His Tag
Sequence Amino acid sequence derived from human IL9 (NP_000581.1) (Gln 19-Il4 144) was expressed and purified, fused with a polyhistidine tag at the C-terminus.
Activity Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5-3 ng/mL.
Protein length The recombinant human IL9 consists of 136 amino acids and predicts a molecular mass of 15.5 kDa. rhIL9 migrates as multiple bands with the molecular mass of 18-25 kDa band in SDS-PAGE under reducing conditions due to different glycosylation.
Preparation method Baculovirus-Insect Cells
Purity > 97 % as determined by SDS-PAGE
Alternative HP40; IL-9; P40; Interleukin-9; IL9

Product Properties

Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Molecular weight 15.5 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL9 protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL9 protein, His Tag product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 9, also known as IL-9, is a cytokine (cell signalling molecule) belonging to the group of interleukins. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. IL-9 is a key molecule that affects differentiation of TH17 cells and Treg function. IL-9 predominantly produced by TH17 cells, synergizes with TGF-β1 to differentiate naïve CD4+ T cells into TH17 cells, while IL-9 secretion by TH17 cells is regulated by IL-23. Interestingly, IL-9 enhances the suppressive functions of FoxP3+ CD4+ Treg cells in vitro, and absence of IL-9 signaling weakens the suppressive activity of nTregs in vivo, leading to an increase in effector cells and worsening of experimental autoimmune encephalomyelitis. The mechanism of IL-9 effects on TH17 and Tregs is through activation of STAT3 and STAT5 signaling. Our findings highlight a role of IL-9 as a regulator of pathogenic versus protective mechanisms of immune responses.
Gene ID 3578
Alternative HP40; IL-9; P40; Interleukin-9; IL9
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession NP_000581.1

Image & description

Fig.SDS-PAGE analysis of Human IL-9 protein, His Tag.

Fig.SDS-PAGE analysis of Human IL-9 protein, His Tag.

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