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Human IL-8 protein (aa 23-99)

Human IL-8 protein (aa 23-99)

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Specification

Product name Human IL-8 protein (aa 23-99)
Sequence The 77 amino acid endothelial-cell derived form (Ala 23-Ser 99) of the mature human IL8 (NP_000575.1) was expressed and purified.
Activity Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 15-75 ng/ml.
Protein length The recombinant mature human cytokine IL8 comprising the 1-77 amino acids predicts a molecular mass of 8 kDa, and migrates as an approximately 12 kDa protein in SDS-PAGE due to glycosylation.
Preparation method HEK293 Cells
Purity > 95 % as determined by SDS-PAGE
Alternative GCP-1; GCP1; IL-8; IL8; Interleukin-8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1; Interleukin-8; CXCL8

Product Properties

Formulation Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5
Molecular weight 8 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL8 protein (aa 23-99) in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL8 protein (aa 23-99) product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.
Gene ID 3576
Alternative GCP-1; GCP1; IL-8; IL8; Interleukin-8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1; Interleukin-8; CXCL8
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession NP_000575.1

Image & description

Fig.SDS-PAGE analysis of Human IL-8 protein (aa 23-99).

Fig.SDS-PAGE analysis of Human IL-8 protein (aa 23-99).

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