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Human IL-5 protein

Human IL-5 protein

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Specification

Product name Human IL-5 protein
Sequence Amino acid sequence derived from human IL5 (P05113) (Ile20-Ser134) was expressed.
Activity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-1.5 ng/ml. The ED50 for this effect is typically 0.1-0.5 ng/ml.
Protein length The recombinant human IL5 consists of 115 amino acids and predicts a molecular mass of 13.1 KDa. It migrates as an approximately 27 KDa band in SDS-PAGE under non-reducing conditions.
Preparation method E. coli
Purity > 95 % as determined by SDS-PAGE
Alternative EDF; IL-5; TRF; Interleukin-5; IL5

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Molecular weight 13.1 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL5 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL5 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 5 (IL-5) is a member of the interleukin family with length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins / signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered as a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues, and can modulate eosinophil behavior at every stage from maturation to survival.
Gene ID 3567
Alternative EDF; IL-5; TRF; Interleukin-5; IL5
Accession P05113

Image & description

Fig.SDS-PAGE analysis of Human IL-5 protein.

Fig.SDS-PAGE analysis of Human IL-5 protein.

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