| Product name | Human IL-4 protein |
| Immunogen | PRP100273 |
| Sequence | Amino acid sequence derived from mature form of human IL-4 isoform 1 (P05112-1) (His 25-Ser 153) was expressed and purified, with an initial Met. |
| Activity | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 × 10E4 IU/μg. |
| Protein length | The recombinant human IL-4 consisting of 130 amino acids and migrates with an apparent molecular mass of 15 kDa as predicted. |
| Preparation method | E. coli |
| Purity | > 92 % as determined by SDS-PAGE |
| Alternative | BCGF-1; BCGF1; BSF-1; BSF1; IL-4; Interleukin-4; Interleukin-4; IL4 |
| Formulation | Lyophilized from sterile 20mM MES, 200mM NaCl, pH 6.0 |
| Molecular weight | 15 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL4 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Human IL-4 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.70% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin-4, also known as IL-4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL-4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL-4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL-4 receptor α, completely inhibites in a dose-dependent manner the IL-4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL-4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL-4-stimulated BA/F3 cells. |
| Gene ID | 3565 |
| Alternative | BCGF-1; BCGF1; BSF-1; BSF1; IL-4; Interleukin-4; Interleukin-4; IL4 |
| Accession | P05112-1 |
Fig.SDS-PAGE analysis of Human IL-4 protein.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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