Login Register
English
0

Cart

$ 0

Human IL-4 protein

Human IL-4 protein

Views(354) Publications(0) Catalog no(PRP100273)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human IL-4 protein
Sequence Amino acid sequence derived from mature form of human IL4 isoform 1 (P05112-1) (His 25-Ser 153) was expressed and purified, with an initial Met.
Activity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 × 10E4 IU/μg.
Protein length The recombinant human IL4 consisting of 130 amino acids and migrates with an apparent molecular mass of 15 kDa as predicted.
Preparation method E. coli
Purity > 92 % as determined by SDS-PAGE
Alternative BCGF-1; BCGF1; BSF-1; BSF1; IL-4; Interleukin-4; Interleukin-4; IL4

Product Properties

Formulation Lyophilized from sterile 20mM MES, 200mM NaCl, pH 6.0
Molecular weight 15 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL4 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL4 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.
Gene ID 3565
Alternative BCGF-1; BCGF1; BSF-1; BSF1; IL-4; Interleukin-4; Interleukin-4; IL4
Accession P05112-1

Image & description

Fig.SDS-PAGE analysis of Human IL-4 protein.

Fig.SDS-PAGE analysis of Human IL-4 protein.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human IL-4 protein”