| Product name | Human IL-35 (IL-12A & IL-27B) protein, Fc Tag |
| Immunogen | PRP100184 |
| Sequence | Amino acid sequence derived from human IL-35 complex composed of IL-27B subunit (NP_005746.2) (Met 1-Lys 229) and the mature form of human IL-12 p35 subunit (P29459) (Arg 23-Ser 219) linked by a polypeptide linker was fused with the Fc region of human IgG1 at the C-terminus. |
| Activity | Measured by its ability to bind biotinylated human IL-6RB-Fch in a functional ELISA. |
| Protein length | The recombinant human IL-35/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 645 amino acids and has a predicted molecular mass of 73.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL-35/Fc monomer is approximately 90-100 kDa due to glycosylation. |
| Preparation method | HEK293 Cells |
| Purity | > 85 % as determined by SDS-PAGE |
| Alternative | CLMF; IL-12A; IL-35; Interleukin-35; NFSK; NKSF1; P35; Interleukin-27 subunit beta & Interleukin-12 subunit alpha; IL35 |
| Formulation | Lyophilized from sterile PBS, pH 7.4 |
| Molecular weight | 73.4 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-35 (IL12A & IL27B) protein, Fc Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Human IL-35 (IL-12A & IL-27B) protein, Fc Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.54% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The novel Ebi3-IL-12α heterodimeric cytokine has been designated interleukin-35 (IL-35), is a member IL-12 family cytokine produced by regulatory T cells (Treg), but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of IL-12α (P35) and IL-27β chains, which are encoded by two separate genes called IL-12A and EBI3 (Epstein-Barr-virus-induced gene 3) respectively. Ectopic expression of IL-35 confers regulatory activity on naive T cells, whereas recombinant IL-35 suppresses T-cell proliferation. It identify IL-35 as a novel inhibitory cytokine that may be specifically produced by T(reg) cells and is required for maximal suppressive activity. IL-35 has biological activity and able to expand CD4+CD25+ Treg cells, suppress the proliferation of CD4+CD25- effector cells and inhibit Th17 cell polarization. IL-35 has been shown to be constitutively expressed by regulatory T (Treg) cells CD4(+)CD25(+)Foxp3(+) and suggested to contribute to their suppressive activity. IL-35 is a crucial mediator which provokes CD4+CD25+ T cell proliferation and IL-10 generation, another well-known anti-inflammatory cytokine, along with TGFβ cytokine. IL-35 is a cytokine can downregulate Th17 cell development and inhibit autoimmune inflammation. It inhibited the differentiation of Th17 cells in vitro. In vivo, IL-35 effectively attenuated established collagen-induced arthritis in mice, with concomitant suppression of IL-17 production but enhanced IFN-gamma synthesis. Thus, IL-35 is a novel anti-inflammatory cytokine suppressing the immune response through the expansion of regulatory T cells and suppression of Th17 cell development. |
| Gene ID | 10148/3592 |
| Alternative | CLMF; IL-12A; IL-35; Interleukin-35; NFSK; NKSF1; P35; Interleukin-27 subunit beta & Interleukin-12 subunit alpha; IL35 |
| Others | The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method |
| Accession | NP_005746.2 & P29459 |
Fig.SDS-PAGE analysis of Human IL-35 (IL-12A & IL-27B) protein, Fc Tag.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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