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Human IL-31 protein, His Tag

Human IL-31 protein, His Tag

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Specification

Product name Human IL-31 protein, His Tag
Sequence Amino acid sequence derived from human IL31 (NP_001014358.1) (Met1-Thr164) was expressed with a polyhistidine tag at the C-terminus.
Activity Measured by its ability to induce STAT3 activation in U‑87 cells. The ED50 for this effect is 11-45 ng/mL. The ED50 for this effect is 11-45 ng/mL.
Protein length The recombinant human IL31 consists of 152 amino acids and predicts a molecular mass of 17.3 KDa. It migrates as an approximately 31.8 KDa band in SDS-PAGE under reducing conditions.
Preparation method HEK293 Cells
Purity > 95 % as determined by SDS-PAGE
Alternative IL-31; Interleukin-31; IL31

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Molecular weight 17.3 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL31 protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL31 protein, His Tag product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IL-31 produced by activated Th2-type T cells, cooperates with a heterodimeric receptor consisting of IL-31 Receptor Anatagonist and Onconstatin-M Receptor that is continuesly expressed on epithelial cells and keratinocytes. IL-31 plays a role in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma. IL-31 is involved in the itching sensation and endorses the scratching behavior in NC/Nga mice with atopic dermatitis. IL-31 expression is connectd with CLA(+) T cells and contributes to the development of atopic dermatitis-induced skin inflammation and pruritus. IL-31 is a powerful inducer of proinflammatory mediators in human colonic SEMFs. IL-31 takes part as a proinflammatory cytokine derived from Th2 cells. Serum IL-31 level is higher in patients with atopic dermatitis. IL-31 is involved in a broad range of immune- & non-immune cells & possesses potential pleiotropic physiological functions, including regulating hematopoiesis & immune response, causing inflammatory bowel disease, airway hypersensitivity & dermatitis.
Gene ID 386653
Alternative IL-31; Interleukin-31; IL31
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession NP_001014358.1

Image & description

Fig.SDS-PAGE analysis of Human IL-31 protein, His Tag.

Fig.SDS-PAGE analysis of Human IL-31 protein, His Tag.

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