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Human IL-18 protein

Human IL-18 protein

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Specification

Product name Human IL-18 protein
Immunogen PRP100039
Sequence Amino acid sequence derived from human IL-18 (NP_001553.1) (Tyr37-Asp193) was expressed.
Protein length The recombinant human IL-18 consists 157 amino acids and predicts a molecular mass of 18.2 kDa.
Preparation method E. coli
Purity > 85 % as determined by SDS-PAGE.
Alternative IGIF; IL-18; IL-1g; IL1F4; Interleukin 18; Interleukin-18; IL18

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Molecular weight 18.2 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL18 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL-18 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.32% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL-12 has been shown to inhibit IL-4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL-18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.
Gene ID 3606
Alternative IGIF; IL-18; IL-1g; IL1F4; Interleukin 18; Interleukin-18; IL18
Accession NP_001553.1

Image & description

Fig.SDS-PAGE analysis of Human IL-18 protein.

Fig.SDS-PAGE analysis of Human IL-18 protein.

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