Login Register
English
0

Cart

$ 0

Human IL-17 protein

Human IL-17 protein

Views(185) Publications(0) Catalog no(PRP100290)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human IL-17 protein
Sequence Amino acid sequence derived from mature form of human IL17A(Q16552) (Ile20-Ala155) was expressed with a N-terminal Met.
Activity Measured by its binding ability in a functional ELISA. Immobilized human IL17A at 10 μg/ml(100 μl/well) can bind human IL17Ra. The EC50 of human IL17Ra is 20.0-48.0 ng/ml.
Protein length The recombinant human IL17A consists of 132 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as an approximately 16, 19, and 20 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
Preparation method CHO Stable Cells
Purity (16.9+21.7+50.1) % as determined by SDS-PAGE
Alternative CTLA-8; CTLA8; IL-17; IL-17A; IL17; Interleukin-17A; IL17A

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Molecular weight 15.7 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL17 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL17 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Gene ID 3605
Alternative CTLA-8; CTLA8; IL-17; IL-17A; IL17; Interleukin-17A; IL17A
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession Q16552

Image & description

Fig.SDS-PAGE analysis of Human IL-17 protein.

Fig.SDS-PAGE analysis of Human IL-17 protein.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human IL-17 protein”