Login Register
English
0

Cart

$ 0

Human IL-15 protein, His Tag

Human IL-15 protein, His Tag

Views(71) Publications(0) Catalog no(PRP100103)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human IL-15 protein, His Tag
Sequence Amino acid sequence derived from human IL15 (NP_000576.1) (Asn18-Ser162) was expressed with a polyhistidine tag at the N-terminus.
Activity Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 10-40 ng/mL.
Protein length The recombinant human IL15 consists of 130 amino acids and predicts a molecular mass of 14.9 KDa. It migrates as an approximately 15 KDa band in SDS-PAGE under reducing conditions.
Preparation method E. coli
Purity > 95 % as determined by SDS-PAGE
Alternative IL-15; Interleukin 15; Interleukin-15; IL15

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Molecular weight 14.9 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL15 protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL15 protein, His Tag product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by IL-15 is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported.
Gene ID 3600
Alternative IL-15; Interleukin 15; Interleukin-15; IL15
Accession NP_000576.1

Image & description

Fig.SDS-PAGE analysis of Human IL-15 protein, His Tag.

Fig.SDS-PAGE analysis of Human IL-15 protein, His Tag.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human IL-15 protein, His Tag”