| Product name | Human IL-15 protein, His Tag |
| Immunogen | PRP100103 |
| Sequence | Amino acid sequence derived from human IL-15 (NP_000576.1) (Asn18-Ser162) was expressed with a polyhistidine tag at the N-terminus. |
| Activity | Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 10-40 ng/mL. |
| Protein length | The recombinant human IL-15 consists of 130 amino acids and predicts a molecular mass of 14.9 KDa. It migrates as an approximately 15 KDa band in SDS-PAGE under reducing conditions. |
| Preparation method | E. coli |
| Purity | > 95 % as determined by SDS-PAGE |
| Alternative | IL-15; Interleukin 15; Interleukin-15; IL15 |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Molecular weight | 14.9 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL15 protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Human IL-15 protein, His Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.39% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The protein encoded by IL-15 is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL-2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported. |
| Gene ID | 3600 |
| Alternative | IL-15; Interleukin 15; Interleukin-15; IL15 |
| Accession | NP_000576.1 |
Fig.SDS-PAGE analysis of Human IL-15 protein, His Tag.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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