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Human IL-10 protein

Human IL-10 protein

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Product name Human IL-10 protein
Immunogen PRP100228
Sequence Amino acid sequence derived from human IL-10 (NP_000563.1) (Ser 19-Asn 178) was expressed.
Activity 1. Immobilized human IL-10 at 10 μg/mL (100 μl/well) can bind Cynomolgus IL-10RA-Fc, The EC50 of Cynomolgus IL-10RA-Fc is 0.24-0.56 μg/mL. 2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 0.2-1.2 ng/mL.
Protein length The recombinant human GADD45G consists of 160 amino acids and predicts a molecular mass of 18.6 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
Preparation method E. coli
Purity > 95 % as determined by SDS-PAGE
Alternative CSIF; GVHDS; IL-10; IL10A; Interleukin-10; TGIF; Interleukin-10; IL10

Product Properties

Formulation Lyophilized from sterile 20mM Tris, 20mM NaCl, pH 8.7.
Molecular weight 18.6 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL10 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL-10 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.60% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IL-10 is a anti-inflammatory cytokine which belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.
Gene ID 3586
Alternative CSIF; GVHDS; IL-10; IL10A; Interleukin-10; TGIF; Interleukin-10; IL10
Accession NP_000563.1

Image & description

Fig.SDS-PAGE analysis of Human IL-10 protein.

Fig.SDS-PAGE analysis of Human IL-10 protein.

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