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Human IL-1 beta protein

Human IL-1 beta protein

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Specification

Product name Human IL-1 beta protein
Sequence Amino acid sequence derived from mature form of human IL1β (NP_000567.1) (Ala 117-Ser 269) was expressed.
Activity 1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 μg/mL (100μl/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml. 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL. 3. Measured by its binding ability in a functional ELISA. 4. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 μg/mL. 5. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.
Protein length The recombinant human IL1β consisting of 154 amino acids migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.
Preparation method E. coli
Purity > 95 % as determined by SDS-PAGE
Alternative IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; Interleukin-1 beta; IL1B

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4
Molecular weight 17 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-1 beta protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL-1 beta protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).
Gene ID 3553
Alternative IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; Interleukin-1 beta; IL1B
Accession NP_000567.1

Image & description

Fig.SDS-PAGE analysis of Human IL-1 beta protein.

Fig.SDS-PAGE analysis of Human IL-1 beta protein.

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