Login Register
English
0

Cart

$ 0

Human IL-1 beta protein (pro form), His Tag

Human IL-1 beta protein (pro form), His Tag

Views(211) Publications(0) Catalog no(PRP100050)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human IL-1 beta protein (pro form), His Tag
Sequence Amino acid sequence derived from pro form of human IL1β (NP_000567.1) (Met 1-Ser 269) was fused with a polyhistidine tag at the N-terminus.
Activity 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1. The EC50 of human IL1R1 is 0.198 μg/mL.
Protein length The recombinant pro form of human IL1β consisting of 280 amino acids and has a calculated molecular mass of 32.3 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions.
Preparation method E. coli
Purity > 95 % as determined by SDS-PAGE
Alternative IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; Interleukin-1 beta; IL1B

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4
Molecular weight 32.3 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-1 beta protein (pro form), His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL-1 beta protein (pro form), His Tag product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).
Gene ID 3553
Alternative IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; Interleukin-1 beta; IL1B
Accession NP_000567.1

Image & description

Fig.SDS-PAGE analysis of Human IL-1 beta protein (pro form), His Tag.

Fig.SDS-PAGE analysis of Human IL-1 beta protein (pro form), His Tag.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human IL-1 beta protein (pro form), His Tag”