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Human IL-1β protein

Human IL-1β protein

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Product name Human IL-1β protein
Immunogen PRP100051
Sequence Amino acid sequence derived from mature form of human IL-1β (NP_000567.1) (Ala 117-Ser 269) was expressed.
Activity 1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-1B at 2 μg/mL (100μl/well) can bind recombinant human IL-1R2 with a linear range of 6.25-400 ng/ml. 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL. 3. Measured by its binding ability in a functional ELISA. 4. Immobilized human IL-1B at 10 μg/mL (100 μl/well) can bind human IL-1R1, The EC50 of human IL-1R1 is 0.48 μg/mL. 5. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.
Protein length The recombinant human IL-1β consisting of 154 amino acids migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.
Preparation method E. coli
Purity > 95 % as determined by SDS-PAGE
Alternative IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; Interleukin-1 beta; IL1B

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4
Molecular weight 17 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-1 beta protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL-1β proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.35% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin-1β (IL-1β or IL-1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL-1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL-1 superfamily consists of three members, IL-1A (IL-1 α), IL-1B (IL-1β), and IL-1 receptor antagonist (IL-1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1β activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL-1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL-2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).
Gene ID 3553
Alternative IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; Interleukin-1 beta; IL1B
Accession NP_000567.1

Image & description

Fig.SDS-PAGE analysis of Human IL-1 beta protein.

Fig.SDS-PAGE analysis of Human IL-1 beta protein.

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