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Human IL-1β protein (pro form), His Tag

Human IL-1β protein (pro form), His Tag

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Specification

Product name Human IL-1β protein (pro form), His Tag
Immunogen PRP100050
Sequence Amino acid sequence derived from pro form of human IL-1β (NP_000567.1) (Met 1-Ser 269) was fused with a polyhistidine tag at the N-terminus.
Activity 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human IL-1B at 10 μg/mL (100 μl/well) can bind human IL-1R1. The EC50 of human IL-1R1 is 0.198 μg/mL.
Protein length The recombinant pro form of human IL-1β consisting of 280 amino acids and has a calculated molecular mass of 32.3 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions.
Preparation method E. coli
Purity > 95 % as determined by SDS-PAGE
Alternative IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; Interleukin-1 beta; IL1B

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4
Molecular weight 32.3 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-1 beta protein (pro form), His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL-1β protein (pro form), His Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.34% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin-1β (IL-1β or IL-1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL-1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL-1 superfamily consists of three members, IL-1A (IL-1 α), IL-1B (IL-1β), and IL-1 receptor antagonist (IL-1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1β activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL-1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL-2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).
Gene ID 3553
Alternative IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; Interleukin-1 beta; IL1B
Accession NP_000567.1

Image & description

Fig.SDS-PAGE analysis of Human IL-1 beta protein (pro form), His Tag.

Fig.SDS-PAGE analysis of Human IL-1 beta protein (pro form), His Tag.

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