| Product name | Human IL-1α protein |
| Immunogen | PRP100048 |
| Sequence | Amino acid sequence derived from the processed form of human IL-1α (NP_000566.3) (Ser 113-Ala 271) was expressed and purified. |
| Activity | Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 0.2-1 ng/mL . |
| Protein length | The recombinant human IL-1α consists of 159 amino acids and has a predicted molecular mass of 18 kDa. As a result of glycosylation, the apparent molecular mass of rhIL-1α is approximately 22 kDa in SDS-PAGE under reducing conditions. |
| Preparation method | HEK293 Cells |
| Purity | > 95 % as determined by SDS-PAGE |
| Alternative | IL-1A; IL-1F1; IL1; IL1-ALPHA; IL1F1; Interleukin-1 alpha; IL1A |
| Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0 |
| Molecular weight | 18 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-1 alpha protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Human IL-1α proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.33% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | IL-1 α is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6 and TNF-α, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-10, IL-4, IL-13 and IL-5, are synthesized from Th2 immune cells. IL-1 α is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 α stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. |
| Gene ID | 3552 |
| Alternative | IL-1A; IL-1F1; IL1; IL1-ALPHA; IL1F1; Interleukin-1 alpha; IL1A |
| Others | The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method |
| Accession | NP_000566.3 |
Fig.SDS-PAGE analysis of Human IL-1 alpha protein.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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