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Human IGF1 protein

Human IGF1 protein

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Specification

Product name Human IGF1 protein
Sequence Amino acid sequence derived from human IGF1 (NP_001104754.1) (Gly49-Ala118) was expressed.
Activity Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is 15-60 ng/mL.
Protein length The recombinant human IGF1 consists 70 amino acids and predicts a molecular mass of 7.7 kDa.
Preparation method Yeast
Purity > 95 % as determined by SDS-PAGE
Alternative IGF-1; IGF-I; IGF1A; IGFI; IGF‑I; MGF; Insulin-like growth factor I; IGF1

Product Properties

Formulation Lyophilized from sterile PBS,pH 7.4.
Molecular weight 7.7 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IGF1 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IGF1 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IGF I, also known as mechano growth factor, somatomedin-C, IGF-I and IGF1, is a secreted protein which belongs to the?insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ) and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
Gene ID 3479
Alternative IGF-1; IGF-I; IGF1A; IGFI; IGF‑I; MGF; Insulin-like growth factor I; IGF1
Accession NP_001104754.1

Image & description

Fig.SDS-PAGE analysis of Human IGF1 protein.

Fig.SDS-PAGE analysis of Human IGF1 protein.

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