- Product name
Human IFN alpha 2 protein
Amino acid sequence derived from human IFNA2 (NP_000596.2) (Cys24-Glu188) was expressed.
Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV). The EC50 for this effect is typically 0.6-4.2pg/mL.
- Protein length
The recombinant human IFNA2 consists 165 amino acids and predicts a molecular mass of 19.3 kDa.
- Preparation method
> 95 % as determined by SDS-PAGE.
Lyophilized from sterile PBS, pH 7.4.
- Molecular weight
- Usage notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IFN alpha 2 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
- Storage instructions
Lyophilized Human IFN alpha 2 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
The product is shipped at ambient temperature.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Human interferon alpha-2 (IFNα2) is a cytokine belonging to the family of type I IFNs. IFNα2 is a protein secreted by cells infected by a virus and acting on other cells to inhibit viral infection. The first description of IFNs as a cellular agent interfering with viral replication was made by Alick Isaacs and Jean Lindenmann in 1957. The history of this finding was recently reviewed. There are 3 types of IFNs: Interferon type I, Interferon type II and Interferon type III. The type II IFN, also called IFNγ, is produced by specific cells of the immune system. Unlike type I and type III IFNs, IFNγ has only a modest role in directly restricting viral infections. Type I and type III IFNs act similarly. However, the action of type III IFNs, also known as IFNλ, is limited to epithelial cells while type I IFNs act on all body's cells.
- Gene ID
- Alternative names
IFN-alphaA; IFNA; IFNA2B; IFNAB; INFA2; Interferon alpha 2; Interferon alpha-2; IFNA2
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Preparation methods for Biochemical
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- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
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- ♦ Handling and storage suggestion for peptides and protein
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Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
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