|Product name||Human Hydatid IgM antibody (Hyd-Ab-IgM) ELISA Kit|
|Applications notes||This Human Hydatid IgM antibody (Hyd-Ab-IgM) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyd-Ab-IgM. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Hyd-Ab-IgM and incubated. The competitive inhibition reaction is launched between with HRP labeled Hyd-Ab-IgM and unlabeled Hyd-Ab-IgM with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Hyd-Ab-IgM in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Hydatid IgM antibody microplate
• Human Hydatid IgM antibody standard
• Human Hydatid IgM antibody detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Hydatid IgM antibody (Hyd-Ab-IgM) ELISA Kit has high sensitivity and excellent specificity for detection of Human Hyd-Ab-IgM. No significant cross-reactivity or interference between Human Hyd-Ab-IgM and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Hydatid disease in man is caused mainly by infection by the larval stage of the dog tapeworm Echinococcus granulosus. It follows accidental ingestion of tapeworm eggs excreted in the faeces of infected dogs. Worldwide, four species of tapeworm are clinically important to man: E. granulosus, Echinococcus multilocularis, Echinococcus vogeli and Echinococcus oligarthrus, but only E. granulosus has been found in the UK. Hydatid disease is one of the most geographically widespread zoonoses. The natural hosts are canine predators, particularly domestic dogs and foxes (mainly the Arctic fox and the red fox). The condition is complicated and expensive to treat, and may require extensive surgery and prolonged drug therapy. The World Health Organization (WHO) aims for an effective disease control strategy by 2018.|
Fig.1. Human Hydatid IgM antibody (Hyd-Ab-IgM) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.