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Human Heme oxygenase 1 (HMOX1) ELISA Kit

Human Heme oxygenase 1 (HMOX1) ELISA Kit

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Specification

Product name Human Heme oxygenase 1 (HMOX1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes Human Heme oxygenase 1 (HMOX1) ELISA Kit employs a two-site sandwich ELISA to quantitate HMOX1 in samples. An antibody specific for HMOX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMOX1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human HMOX1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of HMOX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA

Product Properties

Calibration range 50 pg/mL-800 pg/mL
Limit of detection 5.0 pg/mL
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Image & description

Fig. Abbkine ELISA kit is series of ELISA to quantitate specific protein in samples.

Fig. Abbkine ELISA kit is series of ELISA to quantitate specific protein in samples.

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