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Human GDNF protein

Human GDNF protein

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Specification

Product name Human GDNF protein
Sequence Amino acid sequence derived from mature form of human GDNF (P39905-2) (Arg 83-Ile 185) was expressed and purified, with additional two aa (Gly& Pro) at the N-terminus.
Activity 1. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-15 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 His Chimera. 2. Measured by its ability to bind biotinylated Human GFRa1-His in functional ELISA.
Protein length The recombinant human GDNF consists of 105 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions.
Preparation method HEK293 Cells
Purity > 97 % as determined by SDS-PAGE
Alternative ATF1; ATF2; HFB1-GDNF; HSCR3; Glial cell line-derived neurotrophic factor; GDNF

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4
Molecular weight 15.1 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human GDNF protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human GDNF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Glial cell line-derived neurotrophic factor(GDNF) is an important member of the GDNF family of ligands(GFL). The GDNF family of ligands is comprised by four neurotrophic factors: glial cell line-derived neurotrophic factor (GDNF), neurturin (NRTN), artemin (ARTN), and persephin (PSPN). It has been found that GFLs play a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. As the founding member, GDNF plays a key role in the promotion of the survival of dopaminergic neurons. GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein also promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption. It has been shown that GDNF results in two Parkinson's disease clinical trial and in a number of animal trials. It has been taken as a potent survival factor for central motoneurons.
Gene ID 2668
Alternative ATF1; ATF2; HFB1-GDNF; HSCR3; Glial cell line-derived neurotrophic factor; GDNF
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession P39905-2

Image & description

Fig.SDS-PAGE analysis of Human GDNF protein.

Fig.SDS-PAGE analysis of Human GDNF protein.

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