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Human Gastrokine-2 (GKN2) ELISA Kit

Human Gastrokine-2 (GKN2) ELISA Kit

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Specification

Product name Human Gastrokine-2 (GKN2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Gastrokine-2 (GKN2) ELISA Kit employs a two-site sandwich ELISA to quantitate GKN2 in samples. An antibody specific for GKN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGKN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GKN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GKN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative GKN2; GDDR; PRO813; TFIZ1; VLTI465; blottin; down regulated in gastric cancer GDDR; down-regulated in gastric cancer GDDR; trefoil factor interactions(z) 1

Product Properties

Kit components • Human Gastrokine-2 microplate
• Human Gastrokine-2 standard
• Human Gastrokine-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Gastrokine-2 (GKN2) ELISA Kit has high sensitivity and excellent specificity for detection of Human GKN2. No significant cross-reactivity or interference between Human GKN2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Gastrokine-2 (GKN2) is a secretory peptide of human gastric surface mucous cells (SMCs). It forms disulfide-linked heterodimers with the trefoil factor family (TFF) peptide TFF1. Binding with TFF2 was also reported. Antral SMCs differ from those of the corpus by their TFF3 expression. GKN2 expression along the antral gland axis, to characterize the continuous regeneration of antral glands, and to investigate the interactions of GKN2 with TFF1, TFF2 and mucins.Maturation of antral SMCs occurs stepwise via trans-differentiation of TFF3 expressing progenitor cells. The TFF1-GKN2 heterodimer and TFF2 differ haracteristically by their binding to gastric mucins. This points to different physiological functions of TFF1 and TFF2, the latter maybe acting as a “link peptide” for stabilization of the gastric mucus.
Gene ID 200504
Alternative GKN2; GDDR; PRO813; TFIZ1; VLTI465; blottin; down regulated in gastric cancer GDDR; down-regulated in gastric cancer GDDR; trefoil factor interactions(z) 1
Accession Q86XP6

Image & description

Fig.1. Human Gastrokine-2 (GKN2) Standard Curve.

Fig.1. Human Gastrokine-2 (GKN2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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