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Human Galectin-12 (LGALS12) ELISA Kit

Human Galectin-12 (LGALS12) ELISA Kit

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Specification

Product name Human Galectin-12 (LGALS12) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Galectin-12 (LGALS12) ELISA Kit employs a two-site sandwich ELISA to quantitate LGALS12 in samples. An antibody specific for LGALS12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LGALS12; GALECTIN-12; GRIP1; galectin 12

Product Properties

Kit components • Human Galectin-12 microplate
• Human Galectin-12 standard
• Human Galectin-12 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Galectin-12 (LGALS12) ELISA Kit has high sensitivity and excellent specificity for detection of Human LGALS12. No significant cross-reactivity or interference between Human LGALS12 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Galectins constitute a family of proteins that bind to β-galactoside residues and have diverse physiological functions. Report on the identification of a galectin-like molecule, galectin-12, in a human adipose tissue cDNA library. The protein contained two potential carbohydrate-recognition domains with the second carbohydrate-recognition domain being less conserved compared with other galectins. Galectin-12 mRNA was predominantly expressed in adipose tissue of human and mouse and in differentiated 3T3-L1 adipocytes. Caloric restriction and treatment of obese animals with troglitazone increased galectin-12 mRNA levels and decreased the average size of the cells in adipose tissue.
Gene ID 85329
Alternative LGALS12; GALECTIN-12; GRIP1; galectin 12
Accession Q96DT0

Image & description

Fig.1. Human Galectin-12 (LGALS12) Standard Curve.

Fig.1. Human Galectin-12 (LGALS12) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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