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Human Flt3 ligand protein, His Tag

Human Flt3 ligand protein, His Tag

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Specification

Product name Human Flt3 ligand protein, His Tag
Sequence Amino acid sequence derived from human FLT3LG (P49771-1) (Thr27-Pro185) was expressed, with a polyhistidine tag at the N-terminus.
Activity Measured in a cell proliferation assay using BaF3 mouse pro­B cells transfected with mouse Flt­3. The ED50 for this effect is typically 2-11 ng/mL.
Protein length The recombinant human human FLT3LG consists of 175 amino acids and predicts a molecular mass of 20.2 KDa. It migrates as an approximately 27 KDa band in SDS-PAGE under reducing conditions.
Preparation method Baculovirus-Insect Cells
Purity > 95 % as determined by SDS-PAGE
Alternative FL; FLT3L; FLT3LG; Fms-related tyrosine kinase 3 ligand; FLT3LG

Product Properties

Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Molecular weight 20.2 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human Flt3 ligand protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human Flt3 ligand protein, His Tag product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background FLT3L, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, FLT3L also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 -positive classical DCs and their CD103 -positive tissue counterparts.
Gene ID 2323
Alternative FL; FLT3L; FLT3LG; Fms-related tyrosine kinase 3 ligand; FLT3LG
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession P49771-1

Image & description

Fig.SDS-PAGE analysis of Human Flt3 ligand protein, His Tag.

Fig.SDS-PAGE analysis of Human Flt3 ligand protein, His Tag.

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