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Human FGF21 protein, His Tag

Human FGF21 protein, His Tag

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Specification

Product name Human FGF21 protein, His Tag
Sequence Amino acid sequence derived from mature form of human FGF21 (NP_061986.1) (His 29-Ser 209) was expressed, with a polyhistide tag at the N-terminus.
Activity Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1­1 µg/mL in the presence of 2.5 µg/mL of rmKlotho β.
Protein length The recombinant human FGF21 consisting of 188 amino acids and has a calculated molecular mass of 20.2 kDa. It migrates as an 23 kDa band in SDS-PAGE under reducing conditions as predicted.
Preparation method E. coli
Purity > 95 % as determined by SDS-PAGE
Alternative UNQ3115 / PRO10196; Fibroblast growth factor 21; FGF21

Product Properties

Formulation Lyophilized from 50 mM Tris, 10 % glycerol, 0.05 % Brig 35, pH 8.0
Molecular weight 20.2 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human FGF21 protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human FGF21 protein, His Tag product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Fibroblast growth factor 21 (FGF21) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 has a hydrophobic amino terminus, which is a typical signal sequence, and appears to be a secreted protein. The metabolic regulator fibroblast growth factor 21 (FGF21) has antidiabetic properties in animal models of diabetes and obesity. FGF21 is a novel adipokine associated with obesity-related metabolic complications in humans. The paradoxical increase of serum FGF21 in obese individuals, which may be explained by a compensatory response or resistance to FGF21, warrants further investigation. FGF-21, which we have identified as a novel metabolic factor, exhibits the therapeutic characteristics necessary for an effective treatment of diabetes.
Gene ID 26291
Alternative UNQ3115 / PRO10196; Fibroblast growth factor 21; FGF21
Accession NP_061986.1

Image & description

Fig.SDS-PAGE analysis of Human FGF21 protein, His Tag.

Fig.SDS-PAGE analysis of Human FGF21 protein, His Tag.

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