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Human DnaJ homolog subfamily B member 1 (DNAJB1) ELISA Kit

Human DnaJ homolog subfamily B member 1 (DNAJB1) ELISA Kit

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Specification

Product name Human DnaJ homolog subfamily B member 1 (DNAJB1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human DnaJ homolog subfamily B member 1 (DNAJB1) ELISA Kit employs a two-site sandwich ELISA to quantitate DNAJB1 in samples. An antibody specific for DNAJB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNAJB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNAJB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNAJB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DNAJB1; HSPF1; Hdj1; Hsp40; RSPH16B; Sis1; DnaJ (Hsp40) homolog; subfmaily B; member 1; heat shock 40kD protein 1; radial spoke 16 homolog B

Product Properties

Kit components • Human DnaJ homolog subfamily B member 1 microplate
• Human DnaJ homolog subfamily B member 1 standard
• Human DnaJ homolog subfamily B member 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human DnaJ homolog subfamily B member 1 (DNAJB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DNAJB1. No significant cross-reactivity or interference between Human DNAJB1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Heat shock proteins or HSPs are being synthesized under different kind of stress conditions and act as molecular chaperones for protein molecules. Because these proteins were first found in cells that were exposed to high temperatures, they are called "heat shock proteins" and have been named according to their molecular weights. Hsp40 (heat shock protein 40 kD) is a mammalian heat shock protein. Its prokaryotic homolog is DnaJ.Hsp40 is a family of heat-shock proteins that contain a 70 amino-acid consensus sequence known as the J domain. The J domain of Hsp40 interacts with Hsp70 heat shock proteins. Hsp40 heat-shock proteins play a role in regulating the ATPase activity of Hsp70 heat-shock proteins.
Gene ID 3337
Alternative DNAJB1; HSPF1; Hdj1; Hsp40; RSPH16B; Sis1; DnaJ (Hsp40) homolog; subfmaily B; member 1; heat shock 40kD protein 1; radial spoke 16 homolog B
Accession P25685

Image & description

Fig.1. Human DnaJ homolog subfamily B member 1 (DNAJB1) Standard Curve.

Fig.1. Human DnaJ homolog subfamily B member 1 (DNAJB1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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