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Human Defensin,alpha 1 (DEFA1) ELISA Kit

Human Defensin,alpha 1 (DEFA1) ELISA Kit

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Specification

Product name Human Defensin,alpha 1 (DEFA1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Defensin,alpha 1 (DEFA1) ELISA Kit employs a two-site sandwich ELISA to quantitate DEFA1 in samples. An antibody specific for DEFA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDEFA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DEFA1; DEF1; DEFA2; HNP-1; HP-1; MGC138393; MRS; OTTHUMP00000196017; defensin; alpha 1; myeloid-related sequence; defensin; alpha 2; myeloid-related sequence

Product Properties

Kit components • Human Defensin,alpha 1 microplate
• Human Defensin,alpha 1 standard
• Human Defensin,alpha 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Defensin,alpha 1 (DEFA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DEFA1. No significant cross-reactivity or interference between Human DEFA1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Defensins are cytotoxic peptides and have a well-defined role in host defense. Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8.The defensin, alpha 1 protein, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. It differs from defensin, alpha 3 by only one amino acid.
Gene ID 1667
Alternative DEFA1; DEF1; DEFA2; HNP-1; HP-1; MGC138393; MRS; OTTHUMP00000196017; defensin; alpha 1; myeloid-related sequence; defensin; alpha 2; myeloid-related sequence
Accession P59665

Image & description

Fig.1. Human Defensin,alpha 1 (DEFA1) Standard Curve.

Fig.1. Human Defensin,alpha 1 (DEFA1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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