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Human Collagen,type V,alpha 1 (COL5A1) ELISA Kit

Human Collagen,type V,alpha 1 (COL5A1) ELISA Kit

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Specification

Product name Human Collagen,type V,alpha 1 (COL5A1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Collagen,type V,alpha 1 (COL5A1) ELISA Kit employs a two-site sandwich ELISA to quantitate COL5A1 in samples. An antibody specific for COL5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCOL5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL5A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative COL5A1; RP11-263F14.1; alpha 1 type V collagen

Product Properties

Kit components • Human Collagen,type V,alpha 1 microplate
• Human Collagen,type V,alpha 1 standard
• Human Collagen,type V,alpha 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Collagen,type V,alpha 1 (COL5A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human COL5A1. No significant cross-reactivity or interference between Human COL5A1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Collagen alpha-1(V) chain is alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen.This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.
Gene ID 1289
Alternative COL5A1; RP11-263F14.1; alpha 1 type V collagen
Accession P20908

Image & description

Fig.1. Human Collagen,type V,alpha 1 (COL5A1) Standard Curve.

Fig.1. Human Collagen,type V,alpha 1 (COL5A1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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