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Human CD80/B7-1 protein, His tag (Animal-Free)

Human CD80/B7-1 protein, His tag (Animal-Free)

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Specification

Product name Human CD80/B7-1 protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from human CD80 (P33681-1) (Met1-Asn242) was expressed with a 6 His tag at the C-terminus.
Protein length The recombinant human CD80/B7-1 protein consists of 214 amino acids and predicts a molecular mass of 25.4 KD. It migrates as an approximately 40-60 KD band in SDS-PAGE under reducing conditions due to glycosylation.
Preparation method HEK293 Cells
Purity >95% as determined by SDS-PAGE.
Alternative CD80; Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen; T-lymphocyte activation antigen CD80

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Features & Benefits Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.
Molecular weight 25.4 KD
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD80/B7-1 protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human CD80/B7-1 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses . Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response . Mature mouse B7-1 consists of a 209 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 22 aa transmembrane segment, and a 38 aa cytoplasmic domain . Within the ECD, mouse B7-1 shares 50% and 68% aa sequence identity with human and rat B7-1, respectively. Alternative splicing generates a short isoform that lacks the second Ig-like domain but retains the ability to bind CD28 . Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4 . B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.
Gene ID 941
Alternative CD80; Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen; T-lymphocyte activation antigen CD80
Accession P33681

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