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Human CD7/GP40 protein, His tag (Animal-Free)

Product Information

  • Product name

    Human CD7/GP40 protein, His tag (Animal-Free)

  • Sequence

    Amino acid sequence derived from human CD7 protein (NP_006128.1,Met 1-Pro 180) was expressed with a polyhistidine tag at the C-terminus.

  • Protein length

    The recombinant human CD7/GP40 protein consists of 161 amino acids and predicts a molecular mass of 18 kD.The apparent molecular mass of the human CD7 is approximately 25-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.

  • Preparation method

    HEK293 Cells

  • Purity

    >95% as determined by SDS-PAGE

Fig.SDS-PAGE analysis of Human CD7/GP40 protein

Product Properties

  • Formulation

    0.22 µm filtered protein solution is in PBS, pH 7.4.

  • Features & Benefits

    Endotoxin: <0.1 EU per μg of the protein as determined by the LAL method.

  • Molecular weight

    18 kDa

  • Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human CD7/GP40 protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.

  • Storage instructions

    Lyophilized human CD7/GP40 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

  • Shipping

    The product is shipped at ambient temperature.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    The CD7 antigen is a member of the immunoglobulin superfamily that expresses on the surface of all thymocytes, a majority of mature T cells, and also natural killer cells. Interestingly, under physiological and different pathological conditions, the loss of CD7 antigen occurred in the subset of CD4+ memory T cells. The functions of the cell membrane CD7 antigen have been investigated in human mature T and NK cells, showing the direct involvement of this molecule in multiple effector functions related with activation, proliferation, production of cytokines and modification of adhesion properties. The CD7 glycoprotein is not only expressed by mature lymphoid cells, but also by early hematopoietic progenitors and several types of leukemias, suggesting a role of CD7 during hematopoiesis.

  • Gene ID

    924

  • Alternative names

    CD7 Molecule;T-Cell Leukemia Antigen;T-Cell Antigen CD7;TP41;T-Cell Surface Antigen Leu-9;CD7 Antigen (P41);P41 Protein;LEU-9;Tp40;CD7 Antigen 4;CD7

  • Accession

    P09564

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: PRP2033

  • Size
  • Price
  • 1 mg $1879
  • 10 µg $59
  • 100µg $219
  • 50 µg $149

Contact information

Do not hesitate to contact us if you have any questions.

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