- Product name
Human CD3E protein, Fc tag (Animal-Free)
Amino acid sequence derived from human CD3E protein (P07766, Asp 23-Asp 126) was expressed with the Fc region of human IgG1 at the C-terminus.
- Protein length
The recombinant human CD3E protein consists of 335 amino acids and predicts a molecular mass of 38 kD. The apparent molecular mass of the human CD3E is approximately 40-50 kDa in SDS-PAGE under reducing conditions due to glycosylation.
- Preparation method
HEK 293 Cells
>95% as determined by SDS-PAGE
0.22 µm filtered protein solution is in PBS, pH 7.4.
- Features & Benefits
Endotoxin: <0.1 EU per µg of the protein as determined by the LAL method.
- Molecular weight
- Usage notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human CD3E protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.
- Storage instructions
Lyophilized human CD3E protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
The product is shipped at ambient temperature.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
CD3E is a one-way type I membrane glycoprotein on the surface of t cells. As a Part of the TCR-CD3 complex present on T-lymphocyte cell surface, CD3E plays an essential role in adaptive immune response. CD3E contains one Ig-like domain and one ITAM domain. CD3E together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition with a variety of intracellular signal transduction pathways. Polypeptides play an important role in T cell development. CD3E molecules play a crucial role in T cell development, and CD3e gene deficiency can lead to severe immune deficiency. The CD3E gene is also associated with a woman's susceptibility to type I diabetes. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2. The CD3 co-receptor complex is essential for signal transduction after specific binding of the T-cell receptor (TCR).
- Gene ID
- Alternative names
"CD3e Molecule ;CD3e Antigen, Epsilon Polypeptide (TiT3 Complex);T-Cell Surface Glycoprotein CD3 Epsilon Chain;T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex);T3E;T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3 ;CD3e Antigen;CD3-Epsilon;IMD18 ;TCRE ;CD3E"
Most popular with customers
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
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