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Human CD33/Siglec-3 protein, His tag (Animal-Free)

Human CD33/Siglec-3 protein, His tag (Animal-Free)

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Specification

Product name Human CD33/Siglec-3 protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from human CD33 protein (AAA51948, Met1-His259) was expressed with a polyhistidine tag at the C-terminus.
Protein length The recombinant human CD33/Siglec-3 protein consists of 248 amino acids and predicts a molecular mass of 27.3 kD.The apparent molecular mass of the human CD33 is approximately 40-55 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Preparation method HEK 293 Cells
Purity >95% as determined by SDS-PAGE
Alternative CD33 Molecule;SIGLEC3;Sialic Acid-Binding Ig-Like Lectin 3;Myeloid Cell Surface Antigen CD33;CD33 Antigen (Gp67);SIGLEC-3;Gp67;Sialic Acid Binding Ig-Like Lectin 3;CD33 Molecule Transcript 3;CD33 Antigen

Product Properties

Formulation 0.22 µm filtered protein solution is in PBS, pH 7.4.
Features & Benefits Endotoxin: <0.1 EU per μg of the protein as determined by the LAL method.
Molecular weight 27.3 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human CD33/Siglec-3 protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized humanCD33/Siglec-3 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The transmembrane protein CD33 is a sialic acid-binding immunoglobulin-like lectin that mediate the cell-cell interaction and inhibiting normal functions of immune cells. CD33 (siglec-3), a well-known target in leukemia therapy, is an inhibitory sialoadhesin expressed in human leukocytes of the myeloid lineage and some lymphoid subsets, including NK cells. It may constitute a control mechanism of the innate immune system. In the brain, CD33 is mainly expressed on microglial cells.
Gene ID 945
Alternative CD33 Molecule;SIGLEC3;Sialic Acid-Binding Ig-Like Lectin 3;Myeloid Cell Surface Antigen CD33;CD33 Antigen (Gp67);SIGLEC-3;Gp67;Sialic Acid Binding Ig-Like Lectin 3;CD33 Molecule Transcript 3;CD33 Antigen
Accession P20138

Image & description

Fig.SDS-PAGE analysis of Human CD33/Siglec-3 protein.

Fig.SDS-PAGE analysis of Human CD33/Siglec-3 protein.

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