Chat with us, powered by LiveChat

Human CD24 protein, hFc tag (Animal-Free)

Product Information

  • Product name

    Human CD24 protein, hFc tag (Animal-Free)

  • Sequence

    Amino acid sequence derived from human CD24 (NP_037362.1) (Met 1-Gly 59) was expressed with the Fc region of human IgG1 at the C-terminus.

  • Protein length

    The recombinant human CD24 consists of 270 amino acids and predicts a molecular mass of 29.7 KD. It migrates as an approximately 45-55 KD band in SDS-PAGE under reducing conditions due to glycosylation.

  • Preparation method

    HEK293 Cells

  • Purity

    >95% as determined by SDS-PAGE

Fig. SDS-PAGE analysis of Human CD24 protein, hFc tag.

Product Properties

  • Formulation

    Lyophilized from sterile PBS, pH 7.4.

  • Features & Benefits

    Endotoxin: < 0.1 EU per µg of the protein as determined by the LAL method.

  • Molecular weight

    29.7 KD

  • Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD24 protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

  • Storage instructions

    Lyophilized Human CD24 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

  • Shipping

    The product is shipped at ambient temperature.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 24, also known as signal transducer CD24 or heat stable antigen CD24 (HSA), is a mucin-type glycosylphosphatidylinositol-linked glycoprotein expressed on the surface of B-cells, differentiating neuroblasts and many tumors. May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. Plays a role in the control of autoimmunity.

  • Gene ID

    100133941

  • Alternative names

    Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; CD24; CD24A 

  • Accession

    P25063-1

Most popular with customers

Reviews

There are no reviews yet.

Be the first to review “Human CD24 protein, hFc tag (Animal-Free)”

Your email address will not be published. Required fields are marked *

Human CD24 protein, hFc tag (Animal-Free)
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: PRP2022

  • Size
  • Price
  • 10µg $48
  • 50µg $120
  • 100µg $170

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

Gaining Your Trust with our

High quality *
Attractive price
100% Guarantee
Secured shipment