| Product name | Human CD19 protein, Fc tag (Animal-Free) |
| Sequence | Amino acid sequence derived from human CD19 protein (P15391-1, Pro20-Lys291) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Activity | Immobilized human CD19 at 2 μg/mL (100 μL/well) can bind anti-CD19 antibody, the EC50 for this effect is 1.5 μg/mL. |
| Protein length | The recombinant human CD19 protein consists of 509 amino acids and predicts a molecular mass of 56.9 kD. The apparent molecular mass of the human CD19 is approximately 70-90 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Preparation method | HEK 293 Cells |
| Purity | >90% as determined by SDS-PAGE |
| Alternative | CD19 Molecule;B-Lymphocyte Surface Antigen B4;T-Cell Surface Antigen Leu-12;Differentiation Antigen CD19;B-Lymphocyte Antigen CD19;CD19 Antigen;CVID3;CD19;B4 |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.4. |
| Features & Benefits | Endotoxin: <1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 56.9 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human CD19 protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Human CD19 protein, Fc tag (Animal-Free)should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.109% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | CD19 is a B-lineage-specific transmembrane glycoprotein, the expression of which is maintained on more than 95% B-cell malignancies. This strict lineage restriction makes CD19 an ideal target for immune therapies using chimeric antigen receptors (CARs). In normal cells, it is the most ubiquitously expressed protein in the B lymphocyte lineage. CD19 expression is induced at the point of B lineage commitment during the differentiation of the hematopoietic stem cell, and its expression continues through preB and mature B cell differentiation until it is finally down-regulated during terminal differentiation into plasma cells. CD19 expression is maintained in B-lineage cells that have undergone neoplastic transformation, and therefore CD19 is useful in diagnosis of leukemias and lymphomas using monoclonal antibodies (mAbs) and flow cytometry. |
| Gene ID | 930 |
| Alternative | CD19 Molecule;B-Lymphocyte Surface Antigen B4;T-Cell Surface Antigen Leu-12;Differentiation Antigen CD19;B-Lymphocyte Antigen CD19;CD19 Antigen;CVID3;CD19;B4 |
| Accession | P15391-1 |
Fig.SDS-PAGE analysis of Human CD19 protein.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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