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Human CD19 protein, Fc tag (Animal-Free)

Product Information

  • Product name

    Human CD19 protein, Fc tag (Animal-Free)

  • Sequence

    Amino acid sequence derived from human CD19 protein (P15391-1, Pro20-Lys291) was expressed with the Fc region of human IgG1 at the C-terminus.

  • Protein length

    The recombinant human CD19 protein consists of 509 amino acids and predicts a molecular mass of 56.9 kD. The apparent molecular mass of the human CD19 is approximately 70-90 kDa in SDS-PAGE under reducing conditions due to glycosylation.

  • Preparation method

    HEK 293 Cells

  • Purity

    >90% as determined by SDS-PAGE

Fig.SDS-PAGE analysis of Human CD19 protein.

Product Properties

  • Formulation

    0.22 µm filtered protein solution is in PBS, pH 7.4.

  • Features & Benefits

    Endotoxin: <1 EU per μg of the protein as determined by the LAL method.

  • Molecular weight

    56.9 kDa

  • Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human CD19 protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.

  • Storage instructions

    Lyophilized human CD19 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

  • Shipping

    The product is shipped at ambient temperature.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    CD19 is a B-lineage-specific transmembrane glycoprotein, the expression of which is maintained on more than 95% B-cell malignancies. This strict lineage restriction makes CD19 an ideal target for immune therapies using chimeric antigen receptors (CARs). In normal cells, it is the most ubiquitously expressed protein in the B lymphocyte lineage. CD19 expression is induced at the point of B lineage commitment during the differentiation of the hematopoietic stem cell, and its expression continues through preB and mature B cell differentiation until it is finally down-regulated during terminal differentiation into plasma cells. CD19 expression is maintained in B-lineage cells that have undergone neoplastic transformation, and therefore CD19 is useful in diagnosis of leukemias and lymphomas using monoclonal antibodies (mAbs) and flow cytometry.

  • Gene ID


  • Alternative names

    CD19 Molecule;B-Lymphocyte Surface Antigen B4;T-Cell Surface Antigen Leu-12;Differentiation Antigen CD19;B-Lymphocyte Antigen CD19;CD19 Antigen;CVID3;CD19;B4

  • Accession


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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

Cat #: PRP2030

  • Size
  • Price
  • 1 mg $1879
  • 10 µg $59
  • 100 μg $219
  • 50 µg $149

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer:
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  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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