- Product name
Human CD155/PVR protein, His tag (Animal-Free)
Amino acid sequence derived from human CD155 (NP_006496.3) (Met1-Asn343) was expressed with a 6 His tag at the C-terminus.
- Protein length
The recombinant human CD155 consists of 329 amino acids and predicts a molecular mass of 35.9 KD. It migrates as an approximately 53-70 KD band in SDS-PAGE under reducing conditions due to glycosylation.
- Preparation method
>95% as determined by SDS-PAGE
Lyophilized from sterile PBS, pH 7.4.
- Features & Benefits
Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.
- Molecular weight
- Usage notes
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD155/PVR protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
- Storage instructions
Lyophilized Human CD155 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
The product is shipped at ambient temperature.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
CD155 is a transmembrane adhesion protein that belongs to the immunoglobulin superfamily. It possesses three immunoglobulin-like domains, a transmembrane domain, and the intracellular domain. CD155 is encoded by eight exons and differential mRNA splicing results in four isoforms named PVRα, PVRβ, PVRγ, and PVRδ. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhesion, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.
- Gene ID
- Alternative names
Poliovirus Receptor; Nectin-Like Protein 5; NECL-5; CD155; PVR; PVS
Most popular with customers
Human CD155/PVR protein, His tag (Animal-Free)
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Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact firstname.lastname@example.org for your enquiry.
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through email@example.com if you need more information or support.