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Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) ELISA Kit

Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) ELISA Kit

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Specification

Product name Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) ELISA Kit employs a two-site sandwich ELISA to quantitate BMP15 in samples. An antibody specific for BMP15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBMP15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative BMP15; GDF9B; ODG2; POF4; growth/differentiation factor 9B

Product Properties

Kit components • Human Bone morphogenetic protein 15 microplate
• Human Bone morphogenetic protein 15 standard
• Human Bone morphogenetic protein 15 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) ELISA Kit has high sensitivity and excellent specificity for detection of Human BMP15. No significant cross-reactivity or interference between Human BMP15 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Bone morphogenetic protein 15 is a member of the TGF-β superfamily. It is a paracrine signaling molecule involved in oocyte and follicular development. Using Northern blot analysis, BMP15 has been shown to be exclusively expressed in the ovaries. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The transforming growth factor-beta superfamily includes large families of growth and differentiation factors. It is thought that this protein may be involved in oocyte maturation and follicular development as a homodimer or by forming heterodimers with a related protein, Gdf9. Defects in this gene are the cause of ovarian dysgenesis 2.
Gene ID 353351
Alternative BMP15; GDF9B; ODG2; POF4; growth/differentiation factor 9B
Accession Q6PX77

Image & description

Fig.1. Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) Standard Curve.

Fig.1. Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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