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Human Beta-defensin 115 (DEFB115) ELISA Kit

Human Beta-defensin 115 (DEFB115) ELISA Kit

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Specification

Product name Human Beta-defensin 115 (DEFB115) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Beta-defensin 115 (DEFB115) ELISA Kit employs a two-site sandwich ELISA to quantitate DEFB115 in samples. An antibody specific for DEFB115 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDEFB115 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFB115 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFB115 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative DEFB115; DEFB-15; beta-defensin 115; defensin; beta 15

Product Properties

Kit components • Human Beta-defensin 115 microplate
• Human Beta-defensin 115 standard
• Human Beta-defensin 115 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Beta-defensin 115 (DEFB115) ELISA Kit has high sensitivity and excellent specificity for detection of Human DEFB115. No significant cross-reactivity or interference between Human DEFB115 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions.
Gene ID 245929
Alternative DEFB115; DEFB-15; beta-defensin 115; defensin; beta 15
Accession Q30KQ5

Image & description

Fig.1. Human Beta-defensin 115 (DEFB115) Standard Curve.

Fig.1. Human Beta-defensin 115 (DEFB115) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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