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Human BDNF protein

Human BDNF protein

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Specification

Product name Human BDNF protein
Sequence Amino acid sequence derived from human BDNF (P21237) (Met1-Arg249) was expressed and its mature form was purified. The mature form of human, mouse, rat, cynomolgus and canine BDNF sequences are identical.
Activity 1. Measured by its ability to bind biotinylated human TrkB-His (Cat:10047-H08H) in functional ELISA. 2. Measured by its ability to bind Human TrkB-Fch (Cat:10047-H03H) in functional ELISA. 3. Measured by its ability to bind biotinylated mouse TrkB-His (Cat:50132-M08H) in functional ELISA.
Protein length The recombinant mature form of human BDNF consists of 119 amino acids and predicts a molecular mass of 13.5 KDa.
Preparation method CHO Stable Cells
Purity > 95 % as determined by SDS-PAGE
Alternative Brain-derived neurotrophic factor; BDNF

Product Properties

Formulation Lyophilized from sterile PBS
Molecular weight 13.5 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human BDNF protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human BDNF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background BDNF is a member of the nerve growth factor family. It is highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. It also can be detected in heart, lung, skeletal muscle, testis, prostate and placenta. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. During development, BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. It participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. It functions as the major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Gene ID 12064
Alternative Brain-derived neurotrophic factor; BDNF
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession P21237

Image & description

Fig.SDS-PAGE analysis of Human BDNF protein.

Fig.SDS-PAGE analysis of Human BDNF protein.

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