Human BAFF protein

Product Information

  • Product name

    Human BAFF protein

  • Sequence

    Amino acid sequence derived from soluble form of human BAFF (Q9Y275-1) (Ala 134-Leu 285) was expressed and purified.

  • Activity

    Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is typically 0.4-2 ng/mL in the presence of goat anti-mouse IgM μ chain.

  • Protein length

    The recombinant human BAFF consists of 152 amino acids and has a predicted molecular mass of 17 kDa. In SDS-PAGE under reducing conditions, rhBAFF is migrates as an approximately 19 kDa band.

  • Preparation method

    HEK293 Cells

  • Purity

    > 96 % as determined by SDS-PAGE

Fig.SDS-PAGE analysis of Human BAFF protein.

Product Properties

  • Formulation

    Lyophilized from sterile PBS, pH 7.4

  • Molecular weight

    17 kDa

  • Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human BAFF protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

  • Storage instructions

    Lyophilized Human BAFF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

  • Shipping

    The product is shipped at ambient temperature.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    B lymphocyte stimulator (BLyS), also known as TNFSF13B, CD257 and BAFF, is single-pass type II membrane protein, which belongs to the tumor necrosis factor family. BAFF is abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. BAFF is a cytokine and serves as a ligand for receptors TNFRSF13B (TACI), TNFRSF17 (BCMA), and TNFRSF13C (BAFFR). These receptors is a prominent factor in B cell differentiation, homeostasis, and selection. BLyS levels affect survival signals and selective apoptosis of autoantibody-producing B cells. Thus, it acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells. Overexpression of BLyS in mice can lead to clinical and serological features of systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS). BLyS as an attractive therapeutic target in human rheumatic diseases. The ability of BLyS to regulate both the size and repertoire of the peripheral B cell compartment raises the possibility that BLyS and antagonists thereof may form the basis of a therapeutic trichotomy. As an agonist, BLyS protein may enhance humoral immunity in congenital or acquired immunodeficiencies such as those resulting from viral infection or cancer therapy.

  • Gene ID

    10673

  • Alternative names

    BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; ZTNF4; Tumor necrosis factor ligand superfamily member 13B; TNFSF13B

  • Others

    The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method

  • Accession

    Q9Y275-1

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: PRP100022

  • Size
  • Price
  • 5µg $70
  • 20µg $280
  • 100µg $1030
  • 1mg $6030

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