|Product name||Human Asialoglyco protein receptor (ASGPR) ELISA Kit|
|Applications notes||This Human Asialoglyco protein receptor (ASGPR) ELISA Kit employs a two-site sandwich ELISA to quantitate ASGPR in samples. An antibody specific for ASGPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyASGPR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASGPR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASGPR bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Asialoglyco protein receptor microplate
• Human Asialoglyco protein receptor standard
• Human Asialoglyco protein receptor detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Asialoglyco protein receptor (ASGPR) ELISA Kit has high sensitivity and excellent specificity for detection of Human ASGPR. No significant cross-reactivity or interference between Human ASGPR and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The asialoglycoprotein receptor binds to desialylated (galactosyl-terminal) glycoproteins. It transports these glycoproteins via a series of membrane vesicles and tubules to an acidic-sorting organelle where the receptor and ligand dissociate.The ASGR1 gene encodes a glycoprotein that forms the asialoglycoprotein receptor, also known as the Ashwell receptor, which is specific for desialylated (galactosyl-terminal) glycoproteins and is expressed exclusively in hepatic parenchymal cells. Partially deglycosylated plasma glycoproteins are efficiently and specifically removed from the circulation via this receptor-ligand complex. Many functional studies describing the kinetics of ligand-binding, internalization, and recycling of such receptors have been performed on the human hepatoma cell line HepG2.|
Fig.1. Human Asialoglyco protein receptor (ASGPR) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.