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Human Apoptosis inhibitor 4 (API4) ELISA Kit

Human Apoptosis inhibitor 4 (API4) ELISA Kit

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Specification

Product name Human Apoptosis inhibitor 4 (API4) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Apoptosis inhibitor 4 (API4) ELISA Kit employs a two-site sandwich ELISA to quantitate BIRC5 in samples. An antibody specific for BIRC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBIRC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BIRC5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BIRC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative BIRC5; API4; EPR-1; apoptosis inhibitor 4; baculoviral IAP repeat-containing protein 5; survivin variant 3 alpha

Product Properties

Kit components • Human Apoptosis inhibitor 4 microplate
• Human Apoptosis inhibitor 4 standard
• Human Apoptosis inhibitor 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Apoptosis inhibitor 4 (API4) ELISA Kit has high sensitivity and excellent specificity for detection of Human BIRC5. No significant cross-reactivity or interference between Human BIRC5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Survivin is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain.The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.
Gene ID 332
Alternative BIRC5; API4; EPR-1; apoptosis inhibitor 4; baculoviral IAP repeat-containing protein 5; survivin variant 3 alpha
Accession O15392

Image & description

Fig.1. Human Apoptosis inhibitor 4 (API4) Standard Curve.

Fig.1. Human Apoptosis inhibitor 4 (API4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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