|Product name||Human Antibody to group A streptococcal polysaccharide (ASP) ELISA Kit|
|Applications notes||This Human Antibody to group A streptococcal polysaccharide (ASP) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ASP. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated ASP and incubated. The competitive inhibition reaction is launched between with HRP labeled ASP and unlabeled ASP with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of ASP in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Antibody to group A streptococcal polysaccharide microplate
• Human Antibody to group A streptococcal polysaccharide standard
• Human Antibody to group A streptococcal polysaccharide detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Antibody to group A streptococcal polysaccharide (ASP) ELISA Kit has high sensitivity and excellent specificity for detection of Human ASP. No significant cross-reactivity or interference between Human ASP and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Acylation Stimulating Protein (ASP) is one of the factors that control adipose tissue function. It was initially identified from human plasma, and determined to be a derivative of the 3rd complement component (C3). Arginine removal from activated C3 (C3a) by carboxypeptidase results in C3a-desArg, also known as ASP.Human fat cells have been shown to have mRNA for Adipsin (complement factor D), C3 and factor B, all of which are components necessary to generate C3a, the precursor for ASP. Basal production of ASP in adipocytes is dependent on adipocyte differentiation, with ASP production increasing after 7 days of adipocyte differentiation. While ASP is produced by the interaction of C3, factor B and Adipsin in the alternate complement pathway, it can also be potentially generated by the classical complement pathway as well as the lectin pathway.|
Fig.1. Human Antibody to group A streptococcal polysaccharide (ASP) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.