|Product name||Human Anti-respiratory syncytial virus IgA antibody (RSV-Ab-IgA) ELISA Kit|
|Applications notes||This Human Anti-respiratory syncytial virus IgA antibody (RSV-Ab-IgA) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RSV-Ab-IgA. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated RSV-Ab-IgA and incubated. The competitive inhibition reaction is launched between with HRP labeled RSV-Ab-IgA and unlabeled RSV-Ab-IgA with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RSV-Ab-IgA in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Anti-respiratory syncytial virus IgA antibody microplate
• Human Anti-respiratory syncytial virus IgA antibody standard
• Human Anti-respiratory syncytial virus IgA antibody detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Anti-respiratory syncytial virus IgA antibody (RSV-Ab-IgA) ELISA Kit has high sensitivity and excellent specificity for detection of Human RSV-Ab-IgA. No significant cross-reactivity or interference between Human RSV-Ab-IgA and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Respiratory syncytial virus immune globulin (RSV IG) is a sterilized solution obtained from pooled human blood. It contains the immunoglobulins (or antibodies) to protect against infection from respiratory syncytial virus (RSV), a virus that can cause serious illness in children. Antibodies are substances in the blood that fight infections. RSV IG is used to help prevent or reduce the severity of lower respiratory tract (lung) disease caused by respiratory syncytial virus in children who are at increased risk.|
Fig.1. Human Anti-respiratory syncytial virus IgA antibody (RSV-Ab-IgA) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
Welcome any form of communications, and better service will be provided here.
Address： Bldg C17, Optics Valley International Biomedicine Park, Wuhan, China. 430223