|Product name||Human Anti-CSFV IgG Antibody (CSFV-Ab-IgG) ELISA Kit|
|Applications notes||This Human Anti-CSFV IgG Antibody (CSFV-Ab-IgG) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CSFV-Ab-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated CSFV-Ab-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled CSFV-Ab-IgG and unlabeled CSFV-Ab-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of CSFV-Ab-IgG in the sample. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Anti-CSFV IgG Antibody microplate
• Human Anti-CSFV IgG Antibody standard
• Human Anti-CSFV IgG Antibody detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Anti-CSFV IgG Antibody (CSFV-Ab-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Human CSFV-Ab-IgG. No significant cross-reactivity or interference between Human CSFV-Ab-IgG and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Classical Swine Fever Virus (CSFV), Bovine Viral Diarrhoea Virus (BVDV), and Border Disease Virus (BDV) are the 3 members of the genus Pestivirus within the family Flaviviridae. CSFV causes serious losses in the pig industry since it is highly contagious and pathogenic, and can cause widespread deaths. Pigs infected with CSFV may shed a high amount of virus before showing clinical signs of the disease. If animals survive an acute or sub-acute infection they develop antibodies or become chronically infected, a situation where pigs excrete the virus intermittently or continuously until death. In pregnant sows, CSFV is able to cross the placenta and infect fetuses. It causes abortions, fetal mummification and stillborns; if the infection occurs mid gestation (~50-70 days of pregnancy), weak or persistently viremic piglets can be born. These persistently infected piglets are able to spread the virus, due to the absence of an immune response. Occasionally pigs may be infected with BVDV or BDV. These infections in pigs are usually mild and self-limiting, but it is important to identify them from the CSFV infection.|
Fig.1. Human Anti-CSFV IgG Antibody (CSFV-Ab-IgG) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.